七氟醚对骨肉瘤细胞增殖、侵袭、凋亡及化疗敏感性的影响

Effects of sevoflurane on proliferation, invasion, apoptosis and chemosensitivity of osteosarcoma cells

中文关键词: 七氟醚  骨肉瘤  增殖  侵袭  凋亡  化疗敏感性

英文关键词: Sevoflurane  Osteosarcoma  Proliferation  Invasion  Apoptosis  Chemosensitivity

中文摘要:

      
目的探讨七氟醚对骨肉瘤细胞增殖、侵袭、凋亡及化疗敏感性的影响。
方法 选取骨肉瘤MG63细胞，随机分为四组：对照组、1.7%七氟醚组、3.4%七氟醚组和5.1%七氟醚组，分别为不给予七氟醚、给予1.7%、3.4%和5.1%七氟醚处理。采用MTT法检测细胞增殖，流式细胞术检测细胞凋亡，Transwell细胞侵袭实验检测细胞迁移;给予各组顺铂处理，检测各组细胞凋亡情况。
结果培养24、36和72 h的1.7%七氟醚组、3.4%七氟醚组和5.1%七氟醚OD值明显低于对照组（P＜0.05），培养24、36和72 h的5.1%七氟醚组OD值明显低于1.7%七氟醚组、3.4%七氟醚组（P＜0.05）。四组细胞凋亡率、细胞迁移数差异无统计学意义。顺铂作用后，1.7%七氟醚组、3.4%七氟醚组和5.1%七氟醚组细胞凋亡率明显低于对照组（P＜0.05），5.1%七氟醚组细胞凋亡率明显低于1.7%七氟醚组和3.4%七氟醚组（P＜0.05）。
结论七氟醚可抑制骨肉瘤MG63细胞增殖，对细胞迁移、凋亡无明显影响;七氟醚可降低骨肉瘤MG63细胞对顺铂的敏感性。

英文摘要:

      
Objective To investigate effects of sevoflurane on proliferation, invasion, apoptosis and chemosensitivity of osteosarcoma cells.
Methods The osteosarcoma MG63 cells were randomly divided into control group, 1.7% sevoflurane group, 3.4% sevoflurane group and 5.1% sevoflurane group. The control group did not receive sevoflurane, and the rest were given with 1.7%, 3.4%, and 5.1% concentrations sevoflurane, respectively. Cell proliferation was detected by MTT, cell apoptosis was detected by flow cytometry and cell migration was detected by Transwell cell invasion test. Each group was treated with cisplatin, and the apoptosis of each group was detected.
Results The OD values of 1.7% sevoflurane group, 3.4% sevoflurane group and 5.1% sevoflurane group at 24 h, 36 h and 72 h after cultured were significantly lower than that of the control group (P < 0.05), of which OD values in 5.1% sevoflurane group at 24 h, 36 h and 72 h after cultured were significantly less than 1.7% sevoflurane group, 3.4% sevoflurane group (P < 0.05). There were no significant difference in cell apoptosis rate, cell migration number of the four group. Under the effect of cisplatin, the apoptosis rate in 1.7% sevoflurane group, 3.4% sevoflurane group and 5.1% sevoflurane group were significantly lower than that of the control group (P < 0.05), of which apoptosis cell rate in 5.1% sevoflurane group were significantly lower than that of the 1.7% sevoflurane group and 3.4% sevoflurane group (P < 0.05).
Conclusion Sevoflurane, inhibiting the proliferation of osteosarcoma MG63 cells, has no significant effect on cell migration and apoptosis and reduces the sensitivity of osteosarcoma MG63 cells to cisplatin.