依达拉奉对大鼠小肠缺血-再灌注所致肺损伤的保护作用

Edaravone protects against lung injury induced by intestinal ischemia/reperfusion in rat

中文关键词: 小肠  缺血-再灌注损伤  肺  依达拉奉

英文关键词: Intestine  Ischemia reperfusion  Lung  Edaravone

中文摘要:

      目的 探讨依达拉奉对大鼠小肠缺血-再灌注所致肺损伤的保护作用。方法 雄性SD大鼠18只,随机均分为假手术组(Sham组),缺血-再灌注组(IR组)和依达拉奉组(E组)。Sham组只分离肠系膜上动脉,不做其他处理;IR组分离肠系膜上动脉,从大鼠尾静脉注射与E组等量的生理盐水后,用无创动脉夹夹闭120 min后移去动脉夹,再灌注120 min;E组在缺血-再灌注前静脉注射依达拉奉6 mg/kg。再灌注120 min后采集标本。肺组织HE染色后病理学检测,采集腹主动脉血液检测大鼠血清中TNF-α和IL-6浓度,取肺组织检测髓过氧化物酶（MPO）活性和丙二醛（MDA）浓度。结果 与Sham组比较,IR组肺泡上皮细胞广泛水肿、炎性细胞浸润、肺泡肺萎陷、肺毛细血管扩张出血;E组肺组织病理改变较IR组明显改善,肺泡炎性渗出减少;E组病理评分为（2.1±0.7）分，明显低于IR组的(5.7±1.1）分，IR组病理评分明显高于Sham组的（1.5±0.2）分（P＜0.01)；血清中TNF-α和IL-6的浓度明显少于IR组,肺组织中MPO活性和MDA浓度明显低于IR组(P＜0.01)。结论 依达拉奉能够明显改善小肠缺血-再灌注性肺损伤。

英文摘要:

      Objective Intestinal ischemia-reperfusion (I-R) is a critical and triggering event in the development of distal organ dysfunction, frequently involving the lungs. In this study we investigated the effects of edaravone on the prevention of lung injury induced by intestinal I-R in rats. Methods Eighteen male SD rats were randomly divided into 3 groups: Sham operation group (group Sham), I-R group (group IR) and edaravone group (group E). After injecting 6 mg of edaravone or the same volume of 0.9% sodium chloride, the anterior mesenteric artery was clamped with a noninvasive microvascular clip for 120 min and then reperfusion for 120 min. Sham-operated animals underwent the same surgical procedures without clamping. Lung tissues were collected for pathology tested by HE dyed, blood as collected for the analysis of TNF-α and IL-6 concentration by ELISA, small lung tissues were collected for the analysis of lung myeloperoxidase (MPO) activity and malonialdehyde (MDA) concentration. Results Compared with group Sham, the alveolar epithelial cells in group IR was widespread edema, inflammatory cell infiltration, atectasis, disruption of alveolar, and pulmonary capillary hemorrhage. The pathological changes of lung tissue in group E were significantly improved compared with those in group IR, and that of alveolar inflammatory exudate was decreased. Pathological scoring of group E (2.1±0.7), which was significantly lower than that of group IR (5.7±1.1) and group sham (1.5±0.2) score (P＜0.05), so the concentration of TNF-α, IL-6, MPO activity and MDA concentration of group E were less than those of group IR (P＜0.01). Conclusion Edaravone ameliorated the lung injury induced by intestinal I-R.